The turbine blades of aero-engines are subjected to cyclic high-low cycle loads in actual service environments, which is a typical high-low cycle micro motion fatigue condition. The frequent occurrence of fatigue fracture in turbine blades due to fretting fatigue seriously affects the safe operation of aero-engines. In order to study the high-low cycle fretting fatigue problems of hot end component materials, this paper designs an Inconel718 tenon connection simulation component and conducts high-low cycle composite fretting fatigue tests on it. Five different load/frequency experiment conditions were designed to compare the life distribution of the simulated parts under different conditions. The results show that high/low cycle load has significant influence on fretting fatigue life. With the increase of high/low cycle load or frequency, the fretting fatigue life of joints decreases.