ARP4754A Guidelines for Development of Civil Aircraft and Systems is a recommended and accepted standard for the airworthiness of civil aircraft systems. It provides a structured development process assurance method to identify and correct development errors. Based on the life cycle process and data of civil aircraft system, the implementation of civil aircraft system process assurance based on ARP4754A is analyzed from the goal, plan, review and evidence of process assurance. The application of civil aircraft system process assurance based on ARP4754A is explored from three aspects of process assurance activity types, design quality assurance (DQA) audit and DQA participation. The implementation suggestions are given and verified with the actual project. The results show that the process assurance requirements of civil aircraft system based on ARP4754A are implemented into corresponding activities and the evidence of airworthiness requirements is formed, which effectively ensures the quality and continuous improvement of system process.